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KMID : 0988720090250040361
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Journal of the Korean Academy of Stomatognathic Function and occlusion
2009 Volume.25 No. 4 p.361 ~ p.374
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Shin Hyung-Joo
Kim Dae-Gon
Park Chan-Jin
Cho Lee-Ra
Cha Min-Sang
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Abstract
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For successful osteogenesis around the implants, interaction between implant surface and surrounding tissue is important. Biomechanical bonding and biochemical bonding are considered to influence the response of adherent cells. But the focus has shifted surface chemistry. The purpose of this study is to evaluate the MC3T3-E1 osteoblast like cell responses of magnesium (Mg)?ion implanted titanium surface produced using a plasma source ion implantation method. Commercially pure titanium disc was used as substrates. The discs were prepared to produce four different surface, A: Machine?turned surface, B: Mg?implanted surface, C: sandblasted surface, D: sandblasted and Mg?implanted surface. MC3T3?El osteoblastic like cells were cultured on the disc specimens. Cell adhesion, proliferation, differentiation, and synthesis of extracellular matrix were evaluated. The cell adhesion morphology was evaluated by SEM. RT?PCR assay was used for assessment of cell adhesion, proliferation and differentiation. ALP activity was measured for cell differentiation. The results of this study were as follows: 1. SEM showed that cell on Mg?ion groups was more proliferative than that of non Mg?ion groups. On the machine?turned surface, cell showed some degree of contact guidance in aligning with the machining grooves. 2. In RT PCR analysis, osteonectin and c-fos mRNA were more expressed on sandblasted and Mg?ion implanted group. 3. ALP activity was not significantly different among all groups. Within the limitations of this study, the following conclusions were drawn: It might indicate Mg ion implanted titanium surface induce better bone response than non Mg?ion groups.
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KEYWORD
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Mg ion, Ion implantation, Plasma Source Ion Implantation, Cell response
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